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a worth is somewhat higher for Cd-NagA than for Zn-NagA, as expected with the tendency of zinc to decreased the p
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cat vs. pH profile, an individual ionization is observed for both Zn- or Cd-NagA. This ionization should characterize the protonation of the metallic-certain hydroxide that is certainly used for substrate hydrolysis or, alternatively, the general base which is utilized to deprotonate the metallic-sure h2o molecule before substrate hydrolysis. The p
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Residues Q59 and N61 had been mutated collectively to a set of histidine residues to build an HxH motif that is certainly analogous to that located in NagA from B. subtilis
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